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Better Vaccines from Plants? New Polio Vaccine Could Wipe Out Age-Old Disease
The World Health Organization is seeking alternative vaccines that avoid use of the live virus as part of an international drive to completely eradicate the virus worldwide.
(The UK) — Plants have been used to produce a new vaccine against poliovirus in what is hoped to be a major step towards global eradication of the disease. (Photo Credit: PIXINO)
A cross-cutting team of scientists, including Dr. Johanna Marsian working in Professor George Lomonossoff's Lab at the John Innes Centre, Norwich, has produced the novel vaccine with a method that uses virus-like particles (VLPs) - non-pathogenic mimics of poliovirus which are grown in plants.
Genes that carry information to produce VLPs are infiltrated into the plant tissues. The host plant then reproduces large quantities of them using its own protein expression mechanisms.
Professor Lomonossoff, from the John Innes Centre said: "This is an incredible collaboration involving plant science, animal virology and structural biology. The question for us now is how to scale it up - we don't want to stop at a lab technique."
VLPs look like viruses but are non-infectious. They have been biologically engineered so they do not contain the nucleic acid that allows viruses to replicate. This means that they mimic the behavior of the virus, stimulating the immune system to respond without causing an infection of poliomyelitis.
Laboratory tests demonstrated that the poliovirus mimics provided animals with immunity from the disease paving the way for human vaccines to be produced by plants on a major scale with the input of pharmaceutical industry collaborators.
The breakthrough was made by a consortium funded by the World Health Organization (WHO) which is seeking to eradicate a disease that has been known since antiquity. (Photo Credit: John Innes Centre)
WHO is seeking alternative vaccines that avoid use of the live virus as part of an international drive to completely eradicate the virus worldwide.
A global scourge up to the middle of the last century, poliovirus has been reduced by 99 per cent since 1988 due to the Global Polio Eradication Initiative led by the WHO. Current polio vaccines, however, require the production of huge quantities of the virus. Using the live virus not only represents a risk of the virus escaping, the use of the live attenuated (weakened) virus, effectively maintains polio in the global population.
VLPs were expressed at the John Innes Centre using Hypertrans® transient plant expression system which had previously been developed there. This successful development not only holds promise for the production of vaccines for polio: it could become a frontline diagnostic resource in producing vaccines against other viral outbreaks.
"The beauty of this system of growing non-pathogenic virus mimics in plants, is that it boosts our ability to scale-up the production of vaccine candidates to combat emerging threats to human health," said Professor Lomonossoff.
In the past 20 years plants have become serious competitors to bacteria, insect cells, yeast or mammalian cells as production systems for pharmaceutical materials. They are cost-effective requiring simple nutrients, water, carbon-dioxide and sunlight for efficient growth and the transient expression system can be adjusted rapidly with low costs.
The work at the John Innes Centre furthered work of scientists at the University of Leeds, who first discovered a way of producing the virus-like particles (VLP) using the Hypertrans® expression system.
Despite successes of plant-based expression to produce VLPs of papilloma and hepatitis B viruses, poliovirus VLPs had previously proved too unstable to make practical vaccines using this technique. A problem is that the genetic material which causes replication of the virus and which is therefore absent from the VLPs, also has a role in holding the particles together.
However, teams from The National Institute for Biological Standards and Control, and the University of Leeds identified mutations within protein coats which enabled the production of VLPs which are sufficiently stable to act as vaccines. Experiments at the University of Oxford showed that these were identical to native poliovirus retaining their shape when warmed, and which are effective in protecting animals against poliovirus...
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John Innes Center